McCullagh K G, Duance V C, Bishop K A
J Pathol. 1980 Jan;130(1):45-55. doi: 10.1002/path.1711300107.
Collagen of types I, III and V has been identified and localised in adult human aorta, coronary arteries and atherosclerotic plaques using an indirect immunofluorescent method with specific antibodies to human collagen antigens. The distribution of the three types of collagen was distinct. Type I collagen was found around smooth muscle cells in the aortic media and in large amounts in the atherosclerotic plaque. Type III collagen was found in dense deposits alongside the elastic laminae in the aortic media and in diffuse intimal thickening. In the aortic media, there appeared to be more type III collagen than type I. The reverse was true in advanced atherosclerotic plaques. Type V collagen was distributed throughout the extracellular matrix in the aortic media and in the subendothelial region of plaques. These findings confirm earlier biochemical studies and suggest that a major shift in the nature of collagen synthesis occurs within advanced atherosclerotic plaques.
使用针对人胶原蛋白抗原的特异性抗体的间接免疫荧光法，已在成人主动脉、冠状动脉和动脉粥样硬化斑块中鉴定并定位了I型、III型和V型胶原蛋白。这三种类型胶原蛋白的分布各不相同。I型胶原蛋白在主动脉中膜的平滑肌细胞周围被发现，并且在动脉粥样硬化斑块中大量存在。III型胶原蛋白在主动脉中膜的弹性膜旁的致密沉积物以及弥漫性内膜增厚中被发现。在主动脉中膜，III型胶原蛋白似乎比I型更多。在晚期动脉粥样硬化斑块中情况则相反。V型胶原蛋白分布在主动脉中膜的细胞外基质以及斑块的内皮下区域。这些发现证实了早期的生化研究，并表明在晚期动脉粥样硬化斑块中胶原蛋白合成的性质发生了重大转变。
