Kinetics of locomotion of human granulocytes in a double-filter system. Effect of activating plasma with zymosan and casein.

A new modification of Boyden's millipore filter technique is described in which leukocytes are sandwiched between two filters and allowed to migrate in two directions. This technique offers a better basis for comparison with mathematical models than does single filter methods. Using this technique, casein in low concentrations was leukokinetic in the absence of plasma, but did not show leukotactic activity unless plasma was present. Zymosan-activated plasma was leukokinetic and strongly leukotactic.