一种在光学显微镜和电子显微镜水平上关联皮肤观察结果的简单方法。

A simple method for correlating observations on skin at the light and electron microscopic levels.

作者信息

Dubertret L, Bertaux B, Fosse M, Boulvin F, Touraine R

出版信息

Br J Dermatol. 1980 Feb;102(2):149-54. doi: 10.1111/j.1365-2133.1980.tb05685.x.

DOI:10.1111/j.1365-2133.1980.tb05685.x

Abstract

A method is described which allows the embedding of 6 mm cutaneous punch biopsies for subsequent light and electron microscopic examination. Sections of 20 mm2 cut at 0.5-I micrometer are stained for light microscopy. The subtle tinctorial affinities, which approach those achieved on haematological smears, and the preservation of cellular detail frequently make subsequent ultrastructural examination unnecessary. However, when required, a special technique for trimming and resectioning the tissue blocks allows a good correlation between light and electron microscopic observations.

摘要

本文描述了一种方法，该方法可用于包埋6毫米皮肤打孔活检组织，以便后续进行光镜和电镜检查。将组织切成20平方毫米、厚度为0.5 - 1微米的切片用于光镜染色。其细微的染色亲和力接近血液涂片所达到的效果，且细胞细节保存良好，这常常使得后续的超微结构检查变得不必要。然而，如有需要，一种用于修整和重新切片组织块的特殊技术可使光镜和电镜观察结果具有良好的相关性。