Kirkish L S, Barclay M L, Parra J B, Compton A A, McCann D S
Clin Chem. 1978 Oct;24(10):1830-2.
Conventionally, urinary estrogens are measured to monitor fetal well-being in the third trimester of pregnancy. There are many theoretical and practical advantages to the use of recently introduced plasma estriol assays; consequently, we undertook to compare the results of a spectrophotometric assay for total urinary estrogen with values for plasma total and free (unconjugated) estriol obtained by radioimmunoassay with commercially available kits. The data correlate well, r = 0.70 for plasma total estriol with urinary estrogen (expressed as milligrams of estrogens per gram of creatinine [E/C]), r = 0.79 for plasma free estriol with E/C, and r = 0.71 between the two plasma values. Minute-to-minute fluctuations during 90 min and day-to-day fluctuations followed during five days suggest that fluctuations of up to +/- 45% are within the normal range (means +/- SD of the day-to-day fluctuations were 16.9 +/- 7.7% and 15.6+/- 8.2%). Turn-around time is a minimum of 29 h for the urinary estrogen assay, 4.5 h for the plasma total estriol, and 3 h for the plasma free estriol.
按照惯例，在妊娠晚期通过检测尿雌激素来监测胎儿健康状况。使用最近推出的血浆雌三醇检测方法有许多理论和实际优势；因此，我们进行了一项研究，比较用分光光度法检测总尿雌激素的结果与使用市售试剂盒通过放射免疫法获得的血浆总雌三醇和游离（未结合）雌三醇的值。数据相关性良好，血浆总雌三醇与尿雌激素（以每克肌酐中雌激素毫克数[E/C]表示）的相关系数r = 0.70，血浆游离雌三醇与E/C的相关系数r = 0.79，两个血浆值之间的相关系数r = 0.71。90分钟内的每分钟波动以及五天内的每日波动表明，高达+/- 45%的波动在正常范围内（每日波动的均值+/-标准差分别为16.9 +/- 7.7%和15.6 +/- 8.2%）。尿雌激素检测的周转时间最短为29小时，血浆总雌三醇为4.5小时，血浆游离雌三醇为3小时。
