Ezekwe M O, Martin R J
Horm Metab Res. 1980 Apr;12(4):136-9. doi: 10.1055/s-2007-996225.
Pregnant Yorkshire gilts were allotted to three treatment groups during the third trimester of gestation. One group was injected with alloxan at 70 days of gestation; the second group was injected daily with protamine zinc insulin beginning at 80 days, and the third group received saline injections serving as controls. At 112 days of gestation, the fetuses were delivered alive by hysterectomy, and utilized for analyses. Body composition data indicated a significant increase in percent fat in the diabetic progeny. Liver lipogenic enzyme profiles did not indicate a significant change in activity to account for the increased carcass lipid. In fact, liver lipogenesis from labelled pyruvate was depressed in livers of diabetic progeny. Insulin levels were low in all the groups suggesting that hyperglycemia observed in the progeny of diabetic gilts had little insulin stimulatory action on the fetal pancreatic B-cells. Fructose levels were significantly increased in the progeny of diabetic gilts. Insulin injections during pregnancy did not influence fetal body weight or body compositions. The mechanisms(s) of increased lipid deposition in infants of diabetic mothers (IDM) may involve de novo fatty acid synthesis by adipose cell or direct incorporation of maternal fatty acids into fetal adipose cells.
怀孕的约克夏母猪在妊娠晚期被分配到三个治疗组。一组在妊娠70天时注射四氧嘧啶；第二组从80天开始每天注射精蛋白锌胰岛素，第三组注射生理盐水作为对照。在妊娠112天时，通过子宫切除术使胎儿存活分娩，并用于分析。身体成分数据表明糖尿病后代的脂肪百分比显著增加。肝脏脂肪生成酶谱未显示出活性的显著变化以解释胴体脂质的增加。事实上，糖尿病后代肝脏中来自标记丙酮酸的肝脏脂肪生成受到抑制。所有组的胰岛素水平都很低，这表明糖尿病母猪后代中观察到的高血糖对胎儿胰腺β细胞几乎没有胰岛素刺激作用。糖尿病母猪后代中的果糖水平显著升高。孕期注射胰岛素不影响胎儿体重或身体成分。糖尿病母亲婴儿（IDM）脂质沉积增加的机制可能涉及脂肪细胞从头合成脂肪酸或母体脂肪酸直接掺入胎儿脂肪细胞。
