Al-Gailani M, Garrett J R, Kidd A, Kyriacou K, Leite P
Histochemistry. 1980;66(1):59-74. doi: 10.1007/BF00493246.
The distribution of reaction sites for esteroproteases has been assessed in 'resting' salivary glands from rats, guinea-pigs, cats, dogs and rabbits using the new substrate N-acetyl-L-methionine alpha-naphthyl ester (Lexow et al. 1979). Two main types of activity were observed in the parenchyma. 1) Condensed staining was present on the luminal sides of certain ducts in some, but not all, glands, and this type of staining was always most conspicuous in the submandibular glands of each species. It is likely that this periluminal esteroprotease activity arises from secretory enzymes that will pass into the saliva. Their greater presence in submandibular glands may have association with specialised licking activities. 2) Variable diffuse cytoplasmic staining was present in certain acinar cells from some, but not all, glands. This was most pronounced in the mucous cells of zygomatic glands from dogs. It is considered that the enzymes demonstrated in acinar cells are involved in processing secretory products rather than in being secretory themselves. The possibility that this includes 'signal peptidase' is discussed. Ductal activity in rats, cats and guinea-pigs had some relationship with sites of tryptophan staining but not in rabbits or dogs. Any relationship with acinar staining was less evident. Mast cells, showing strong esteroprotease staining, were present in variable numbers in the different glands. Preliminary attempts to qualify the enzymes being demonstrated were made by using the inhibitor E600. This substance inhibited all activity in acinar cells from each species, and in rabbits and dogs it also inhibited the ductal activity as well. However, in rats and guinea-pigs it had little or no effect on the ductal activity and in cats it had only a small inhibitory effect on the ductal activity. E600 had no obvious inhibitory effects on mast cell activity.
利用新型底物N-乙酰-L-蛋氨酸α-萘酯（Lexow等人，1979年），对大鼠、豚鼠、猫、狗和兔子的“静止”唾液腺中酯蛋白酶的反应位点分布进行了评估。在实质组织中观察到两种主要类型的活性。1）在某些（但并非所有）腺体的特定导管腔面存在浓缩染色，并且这种染色类型在每个物种的下颌下腺中总是最为明显。这种管腔周围酯蛋白酶活性可能源于会进入唾液的分泌酶。它们在下颌下腺中含量更高可能与特殊的舔舐活动有关。2）在某些（但并非所有）腺体的特定腺泡细胞中存在可变的弥漫性细胞质染色。这在狗的颧腺黏液细胞中最为明显。据认为，腺泡细胞中显示出的酶参与分泌产物的加工，而不是其本身的分泌。文中讨论了其中包括“信号肽酶”的可能性。大鼠、猫和豚鼠的导管活性与色氨酸染色位点有一定关系，但兔子和狗则不然。与腺泡染色的任何关系不太明显。不同腺体中肥大细胞数量不等，均显示出强烈的酯蛋白酶染色。通过使用抑制剂E600对所显示的酶进行初步定性尝试。该物质抑制了每个物种腺泡细胞中的所有活性，在兔子和狗中它还抑制了导管活性。然而，在大鼠和豚鼠中它对导管活性几乎没有影响，在猫中它对导管活性只有很小的抑制作用。E600对肥大细胞活性没有明显的抑制作用。
