Kyriacou K, Garrett J R
Histochem J. 1985 Jun;17(6):683-98. doi: 10.1007/BF01003520.
The distribution of several hydrolytic enzymes was investigated in rabbit submandibular glands at both the light and electron microscopical levels. Glands were fixed by either immersion or perfusion fixation with a variety of fixatives containing 1-2% glutaraldehyde and 2-4% formaldehyde in 0.1 M cacodylate buffer at pH 7.2. Light microscopically, the acinar cells showed some staining for ATPase, acid phosphatase and nonspecific esterases but showed weak staining for thiamine pyrophosphatase. Acid phosphatase staining occurred most strongly in granular tubule cells. Staining for esteroproteases was confined to the periluminal rims of intercalary and striated ducts. Alkaline phosphatase was very sensitive to glutaraldehyde and was confined to myoepithelial cells. Electron microscopical observations revealed the presence of acid phosphatase reaction product in lysosomes, immature granules and in GERL-like structures, the last being much more conspicuous in the granular tubule cells. ATPase reaction product was localized to the basal and luminal plasma membranes and lumina of both acinar and granular tubule cells. The Golgi complex of these two types of cells exhibited only moderate amounts of reaction product for thiamine pyrophosphatase. Alkaline phosphatase activity, on the other hand, was exclusively localized to myoepithelial cells in their plasma membranes and sometimes in the nuclear envelope.
在光学显微镜和电子显微镜水平上，研究了几种水解酶在兔下颌下腺中的分布。腺体通过浸泡或灌注固定，使用多种固定剂，这些固定剂在pH 7.2的0.1 M二甲胂酸盐缓冲液中含有1-2%的戊二醛和2-4%的甲醛。在光学显微镜下，腺泡细胞对ATP酶、酸性磷酸酶和非特异性酯酶有一些染色，但对硫胺素焦磷酸酶染色较弱。酸性磷酸酶染色在颗粒小管细胞中最为强烈。酯蛋白酶染色局限于闰管和纹状管的管周缘。碱性磷酸酶对戊二醛非常敏感，局限于肌上皮细胞。电子显微镜观察显示，酸性磷酸酶反应产物存在于溶酶体、未成熟颗粒和类GERL结构中，后者在颗粒小管细胞中更为明显。ATP酶反应产物定位于腺泡细胞和颗粒小管细胞的基底和管腔质膜以及管腔。这两种类型细胞的高尔基体对硫胺素焦磷酸酶仅表现出适量的反应产物。另一方面，碱性磷酸酶活性仅定位于肌上皮细胞的质膜，有时也定位于核膜。
