Reynolds G T
Microsc Acta. 1980 Mar;83(1):55-62.
Microscopic observations of weak fluorescence from living cells can be achieved by using image intensification techniques in situations where conventional film recording is not feasible. A brief description is given of experimental arrangements that have been used, involving recording the intensifier output alternately on film, or TV vidicons. References are given to more detailed descriptions of particular systems, and an example is presented of the detection of Ca++ in Haemanthus by means of the fluorescence of chlorotetracycline.
在传统胶片记录不可行的情况下,利用图像增强技术可以实现对活细胞微弱荧光的显微镜观察。文中简要描述了所采用的实验装置,包括交替在胶片或电视摄像管上记录增强器输出。文中还给出了特定系统更详细描述的参考文献,并给出了一个通过金盏花中氯四环素的荧光检测钙离子的实例。