Müller K M
Microsc Acta. 1980 May;83(2):127-30.
Histologic work-up of tissue specimens, especially from endoscopic biopsies, requires the use of fine forceps for orientation and dressing in fluid paraffin during the embedding procedure. These forceps are usually preheated over an open flame. If, however, smaller tissue particles are handled with overheated forceps, arteficial alterations may occur which are apt to hamper or falsify the histologic evaluation of the prepared section. Some typical tissue artefacts due to the handling with overheated forceps, are demonstrated with slides from liver biopsies. Recent experiences with an auxiliary instrument for paraffin embedding (Histostat of Vogel, designed by Ciplea) are reported. The forceps are kept at stable temperatures by immersion in fluid paraffin during the embedding procedure, thus excluding almost completely the risk of tissue artefacts by handling with overheated forceps.
组织标本的组织学检查，尤其是内镜活检标本，在包埋过程中需要使用精细镊子进行定位并在液体石蜡中修整。这些镊子通常在明火上预热。然而，如果用过热的镊子处理较小的组织颗粒，可能会出现人为改变，这易于妨碍或歪曲制备切片的组织学评估。通过肝活检切片展示了一些因使用过热镊子处理而导致的典型组织假象。报告了一种用于石蜡包埋的辅助仪器（由西普勒设计的沃格尔组织包埋机）的近期使用经验。在包埋过程中，通过将镊子浸入液体石蜡中使其保持稳定温度，从而几乎完全排除了因使用过热镊子处理而导致组织假象的风险。
