Oluwole S, Hardy M A, Wang T, Satake K, Todd G, Reemtsma K, Nowygrod R
Transplantation. 1980 Jul;30(1):31-3.
The present experiment was designed to study the relationship between rat heart allograft survival and passenger leukocyte depletion in donor-pretreated animals. Untreated Lewis rats served as recipients of cardiac allografts from treated Fischer rats. Passenger leukocyte depletion was assayed with indium-111 oxine-labeled leukocytes (predominantly lymphocytes) which were infused into donor rats 6 hr before treatment with cyclophosphamide, antilymphocyte globulin (ALG), sublethal total body irradiation, or in vitro perfusion-preservation of the isolated beating heart. In vivo pretreatment of the donor with cyclophosphamide resulted in a significant prolongation of heart allograft survival but effected no reduction in graft-labeled lymphocytes. In vitro perfusion-preservation of the donor heart, for 1 to 2 hr, led to a 50 to 60% reduction in graft-labeled lymphocytes but failed to significantly prolong the survival of the heart allografts. Both ALG and sublethal total body irradiation donor pretreatments resulted in significant prolongation of heart allograft survival and a 20 to 25% labeled passenger lymphocyte depletion. This study demonstrates that there is no direct correlation between allograft survival and the degree of mobile passenger lymphocyte depletion, suggesting that the efficacy of leukocytotoxic donor pretreatment methods may depend in part on alternative mechanisms.
本实验旨在研究供体预处理动物中大鼠心脏同种异体移植存活与过客白细胞清除之间的关系。未经处理的Lewis大鼠作为接受来自经处理的Fischer大鼠心脏同种异体移植的受体。用铟-111氧嗪标记的白细胞（主要是淋巴细胞）检测过客白细胞清除情况，这些白细胞在给予环磷酰胺、抗淋巴细胞球蛋白（ALG）、亚致死剂量全身照射或离体跳动心脏的体外灌注保存处理前6小时注入供体大鼠。供体在体内用环磷酰胺预处理可显著延长心脏同种异体移植的存活时间，但对移植组织中标记的淋巴细胞数量没有减少作用。供体心脏进行1至2小时的体外灌注保存可使移植组织中标记的淋巴细胞数量减少50%至60%，但未能显著延长心脏同种异体移植的存活时间。ALG和亚致死剂量全身照射这两种供体预处理方法均能显著延长心脏同种异体移植的存活时间，并使标记的过客淋巴细胞减少20%至25%。本研究表明，同种异体移植存活与可移动过客淋巴细胞清除程度之间没有直接相关性，这表明白细胞毒性供体预处理方法的疗效可能部分取决于其他机制。
