Belldegrün A, Cohen I R
Transplantation. 1980 Jul;30(1):40-2. doi: 10.1097/00007890-198007000-00008.
Experiments were done to prolong the survival of transplanted allogeneic skin in mice. We used the recruitment model of cell-mediated immunity to remove effector lymphocytes of recipients specifically directed against foreign antigens of the skin graft donor. BALB/c recipient mice were inoculated in both footpads with syngeneic initiator T lymphocytes (ITLs) sensitized against C57BL/6 fibroblasts. At the height of the recruitment response, the reacting popliteal lymph nodes (PLNs), containing specific anti-C57BL/6 effector lymphocytes, were surgically excised. The mice were then engrafted with skin from C57BL/6 mice or third-party C3H/eB mice. We found that selective treatment of the recipient significantly prolonged the mean survival time of the antigen-specific C57BL/6 grafts from 11.9 to 22.1 days. These findings show that physiological immunosuppression can be achieved, without any harmful or toxic side effects, by sequestration of the recipient's specific lymphocytes.
进行了实验以延长小鼠同种异体移植皮肤的存活时间。我们使用细胞介导免疫的募集模型来特异性去除受体中针对皮肤移植供体的外来抗原的效应淋巴细胞。将对C57BL/6成纤维细胞致敏的同基因起始T淋巴细胞（ITL）接种到BALB/c受体小鼠的双足垫中。在募集反应的高峰期，手术切除含有特异性抗C57BL/6效应淋巴细胞的反应性腘淋巴结（PLN）。然后将这些小鼠用来自C57BL/6小鼠或第三方C3H/eB小鼠的皮肤进行移植。我们发现对受体进行选择性治疗可将抗原特异性C57BL/6移植物的平均存活时间从11.9天显著延长至22.1天。这些发现表明，通过隔离受体的特异性淋巴细胞，可以实现生理性免疫抑制，且无任何有害或毒性副作用。
