Gagliardino J J, Pessacq M T, Turyn D, Dellacha J M
Horm Metab Res. 1980 Jul;12(7):300-3. doi: 10.1055/s-2007-996275.
Insulin binding to rat liver cells was studied in vivo, by I. V. injection of (125I)-labelled hormone either alone or together with different amounts of unlabelled insulin. Native insulin competes with the tracer in a manner similar to the one observed in the in vitro preparation. Prolactin, bovine growth hormone or glucagon were unable to displace labelled insulin from its binding sites. The hypoglycemic effect of various amounts of native insulin was measured and correlated to the binding studies. It was found that maximal insulin-induced hypoglycemia occurs at an insulin concentration at which only about 12% of the measurable receptors are occupied.
通过静脉注射（125I）标记的激素单独或与不同量的未标记胰岛素一起，在体内研究了胰岛素与大鼠肝细胞的结合。天然胰岛素与示踪剂的竞争方式与在体外制剂中观察到的相似。催乳素、牛生长激素或胰高血糖素不能从其结合位点取代标记的胰岛素。测量了不同量天然胰岛素的降血糖作用，并将其与结合研究相关联。发现最大胰岛素诱导的低血糖发生在胰岛素浓度下，此时仅约12%的可测量受体被占据。
