粪便中大肠杆菌肠毒素的检测
Detection of Escherichia coli enterotoxins in stools.
作者信息
Merson M H, Yolken R H, Sack R B, Froehlich J L, Greenberg H B, Huq I, Black R W
出版信息
Infect Immun. 1980 Jul;29(1):108-13. doi: 10.1128/iai.29.1.108-113.1980.
Abstract
We determined whether enterotoxigenic Escherichia coli diarrhea could be diagnosed by direct examination of stools for heat-labile (LT) and heat-stable (ST) enterotoxins. The Y-1 adrenal cell and an enzyme-linked immunosorbent assay (ELISA) detected LT in 85 and 93%, respectively, of stool specimens obtained from adults with acute diarrhea from whom an LT- and ST-producing organism had been isolated. Furthermore, the ELISA assay detected LT in 8 of 35 stool specimens from which no LT-producing E. coli had been isolated. The infant mouse assay was utilized to detect ST in these stool specimens and was found to be an insensitive method, showing positive results in only 36% of the specimens from which an ST-producing organism was isolated. Further studies are warranted to determine the diagnostic value of direct detection of LT in stools, especially by the ELISA method.
摘要
我们确定了是否可以通过直接检测粪便中的不耐热（LT）和耐热（ST）肠毒素来诊断产肠毒素大肠杆菌腹泻。Y-1肾上腺细胞和酶联免疫吸附测定（ELISA）分别在从患有急性腹泻的成年人中分离出产生LT和ST的生物体的粪便标本中检测到85%和93%的LT。此外，ELISA检测在35份未分离出产LT大肠杆菌的粪便标本中的8份中检测到LT。采用幼鼠试验检测这些粪便标本中的ST，发现该方法不敏感，仅在36%分离出产ST生物体的标本中显示阳性结果。有必要进一步研究以确定直接检测粪便中LT的诊断价值，尤其是通过ELISA方法。
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