δ5,3-酮类固醇异构酶与人类胎盘催乳素通过分子间二硫键形成进行偶联。该偶联物在灵敏酶免疫测定中的应用。

Coupling of delta 5,3-ketosteroid isomerase to human placental lactogen with intermolecular disulfide bond formation. Use of this conjugate for a sensitive enzyme immunoassay.

作者信息

Terouanne B, Nicolas J C, Descomps B, Crastes de Paulet A

出版信息

J Immunol Methods. 1980;35(3-4):267-75. doi: 10.1016/0022-1759(80)90253-7.

Abstract

An immunoassay for human placental lactogen (HPL) has been developed with the use of hormone labelled with delta 5,3-ketosteroid isomerase. The enzyme is coupled to the antigen via an intermolecular disulfide interchange reaction. HPL-enzyme conjugates are prepared in high yield and purified by affinity chromatography. An enzyme immunoassay using these hormone-enzyme derivatives is compared with radioimmunoassay for determination of HPL in serum.

摘要

利用用δ5,3-酮类固醇异构酶标记的激素开发了一种人胎盘催乳素（HPL）免疫测定法。该酶通过分子间二硫键交换反应与抗原偶联。HPL-酶偶联物以高产率制备并通过亲和层析纯化。将使用这些激素-酶衍生物的酶免疫测定法与放射免疫测定法进行比较，以测定血清中的HPL。