McLeish K R, Gohara A F, Gunning W T, Senitzer D
J Lab Clin Med. 1980 Sep;96(3):470-9.
We studied the effect of PGE1 in pharmacologic doses on immune complex-induced glomerulonephritis produced by daily intraperitoneal injections of apoferritin. Mice received one of the following injection schedules: apoferritin 4 mg/day, apoferritin 4 mg/day plus PGE1 200 microgram twice daily, saline, or PGE1 200 microgram twice daily. Administration of apoferritin alone resulted in mesangial cell proliferation in all 14 mice with crescent formation in nine. Evidence of subepithelial and mesangial immune complex deposition and a significant increase in urine protein excretion was found. Treatment with PGE1 resulted in a mild increase in mesangial cells in six of 14 mice. No mice developed crescents on this regimen. In addition, proteinuria was prevented, and there was a marked diminution of immune complex deposition. Antiapoferritin antibody was detected in the sera of mice from both groups. No alteration in lymphocyte response to mitogen or in vitro PGE1 suppression of blastogenesis was detected. Our results indicate that PGE1 therapy alters immune complex glomerulonephritis in this model of murine chronic serum sickness by reducing glomerular immune complex deposition. However, no difference in specific or nonspecific immunologic responses was detected.
我们研究了药理剂量的前列腺素E1（PGE1）对每日腹腔注射脱铁铁蛋白所致免疫复合物性肾小球肾炎的影响。小鼠接受以下注射方案之一：每天注射4毫克脱铁铁蛋白、每天注射4毫克脱铁铁蛋白加每天两次注射200微克PGE1、注射生理盐水或每天两次注射200微克PGE1。单独给予脱铁铁蛋白导致所有14只小鼠出现系膜细胞增殖，其中9只形成新月体。发现有上皮下和系膜免疫复合物沉积的证据，且尿蛋白排泄显著增加。用PGE1治疗导致14只小鼠中有6只系膜细胞轻度增加。在该治疗方案下无小鼠形成新月体。此外，蛋白尿得到预防，免疫复合物沉积明显减少。在两组小鼠的血清中均检测到抗脱铁铁蛋白抗体。未检测到淋巴细胞对有丝分裂原的反应或体外PGE1对细胞增殖的抑制作用有改变。我们的结果表明，在这种小鼠慢性血清病模型中，PGE1治疗通过减少肾小球免疫复合物沉积来改变免疫复合物性肾小球肾炎。然而，未检测到特异性或非特异性免疫反应的差异。
