Horuk R, Blundell T L, Lazarus N R, Neville R W, Stone D, Wollmer A
Nature. 1980 Aug 21;286(5775):822-4. doi: 10.1038/286822a0.
The insulins of New World hystricomorph rodents exhibit many novel amino acid changes in primary structure when compared with other mammalian insulins. These changes give rise to unusual properties (low potency, failure to self-associate) not shared by other naturally-occurring insulins. We report here on the primary structure, zinc-binding properties and circular dichroism (CD) of porcupine-insulin (Hystrix cristata), the first Old World hystricomorph insulin to be investigated, and discuss the changes in primary structure of the hormone in relation to its properties. Residue B22 is strongly implicated as being responsible for the unusual properties of porcupine insulin.
与其他哺乳动物胰岛素相比，新大陆豪猪型啮齿动物的胰岛素在一级结构上表现出许多新的氨基酸变化。这些变化产生了其他天然存在的胰岛素所没有的异常特性（低效价、无法自我缔合）。我们在此报告豪猪胰岛素（非洲冕豪猪）的一级结构、锌结合特性和圆二色性（CD），这是首个被研究的旧大陆豪猪型胰岛素，并讨论该激素一级结构的变化与其特性的关系。B22残基被强烈认为是豪猪胰岛素异常特性的原因。
