Thompson D A
Ann Clin Biochem. 1980 May;17(3):144-7. doi: 10.1177/000456328001700308.
A flameless atomic absorption spectroscopy method has been developed for the measurement of chromium. Background correction has been modified by recording non-atomic absorption separately from atomic absorption on a double-pen recorder. This was achieved by using the prime spectral line of uranium (358.5 nm, within 0.6 nm of that of chromium--357.9 nm) and was possible only because of the absence of uranium from the plasma samples. Masking of the analytical signal at atomisation by non-atomic absorption was thus eliminated and gave the method a sensitivity of 3 pg and a detection limit of 150 pg/ml. Postprandrial plasma chromium values were measured in 32 insulin-treated diabetics (1.65 +/- 0.15 ng/ml), in 53 non-insulin treated diabetics (2.0 +/- 0.16 ng/ml), and in 144 control non-diabetics (2.00 +/- 0.14 ng/ml). The difference between the mean plasma chromium level of the insulin-treated diabetics and both the non-insulin treated diabetics and the control groups was statistically significant (P < 0.05).
已开发出一种无火焰原子吸收光谱法用于测定铬。通过在双笔记录仪上分别记录非原子吸收和原子吸收来改进背景校正。这是通过使用铀的主谱线（358.5nm，在铬的谱线357.9nm的0.6nm范围内）实现的，并且仅因为血浆样品中不含铀才有可能。这样就消除了原子化时非原子吸收对分析信号的掩蔽，使该方法的灵敏度达到3pg，检测限为150pg/ml。测定了32名接受胰岛素治疗的糖尿病患者（1.65±0.15ng/ml）、53名未接受胰岛素治疗的糖尿病患者（2.0±0.16ng/ml）和144名对照非糖尿病患者（2.00±0.14ng/ml）的餐后血浆铬值。接受胰岛素治疗的糖尿病患者的平均血浆铬水平与未接受胰岛素治疗的糖尿病患者及对照组之间的差异具有统计学意义（P<0.05）。
