Girshovich A S, Dondon J, Grunberg-Manago M
Biochimie. 1980;62(7):509-12. doi: 10.1016/s0300-9084(80)80071-x.
A photoactivated radioactive derivative of IF2 was obtained by treatment with p-azidobenzaldehyde in the presence of NaBH4, followed by reductive methylation with [14C] formaldehyde. The modified IF2 preserved the functional activity of the native factor in specific binding to 30S ribosomal subparticles to which it specifically cross-linked during mild irradiation. The main affinity-labelled component of the 30S subparticle was found to be 16S RNA.
通过在硼氢化钠存在下用对叠氮苯甲醛处理，然后用[14C]甲醛进行还原甲基化，得到了IF2的光活化放射性衍生物。修饰后的IF2在与30S核糖体亚颗粒特异性结合时保留了天然因子的功能活性，在轻度照射下它会与之特异性交联。发现30S亚颗粒的主要亲和标记成分是16S RNA。
