Groner W, Mohandas N, Bessis M
Clin Chem. 1980 Sep;26(10):1435-42.
The laser light scattered by erythrocytes subjected to a well-defined shear stess can be analyzed with the ektacytometer to obtain information regarding the changes in cell shape due to fluid shear. We describe an optical technique whereby an observed quantitative output derived from a mesurement of light intensity through a spatial filter is related to the change in cellular dimensions that were previously observed under similar fluid-shear conditions by use of microscopy and a cone-plate viscometer (rheoscope). We also present the predictions of a theoretical model (of the ektacytometer) based on approximations of light-scattering theory developed for nonspherical particles, and give preliminary results for the accuracy and sensitivity of this measurement of erythrocyte deformability. With this optical technique the instrumentation (ektacytometer) is made quite simple and suitable for use in the typical laboratory. This would allow a regular, quantitative assessment of this important blood cell quality, to supplement the data obtained from the complete blood count.
通过血细胞变形仪可以分析受到明确剪切应力作用的红细胞散射的激光，以获取有关因流体剪切导致的细胞形状变化的信息。我们描述了一种光学技术，通过该技术，从通过空间滤波器测量光强度得出的观察到的定量输出与先前在类似流体剪切条件下使用显微镜和锥板粘度计（流变仪）观察到的细胞尺寸变化相关。我们还基于为非球形颗粒开发的光散射理论的近似值，给出了（血细胞变形仪的）理论模型的预测，并给出了这种红细胞变形性测量的准确性和灵敏度的初步结果。利用这种光学技术，仪器（血细胞变形仪）变得相当简单，适合在典型实验室中使用。这将允许对这种重要的血细胞质量进行常规的定量评估，以补充从全血细胞计数获得的数据。
