Lehmann F G, Cramer P, Hillert U
Clin Chim Acta. 1980 Aug 19;105(3):367-76. doi: 10.1016/0009-8981(80)90117-5.
An immunoprecipitation method for the determination of human intestinal alkaline phosphatase (I-AP) in feces is described using anti-human I-AP IgG. Antibodies to human I-AP are monospecific after absorption with human placental alkaline phosphatase and show no cross-reactions with human liver alkaline phosphatase or alkaline phosphatase from E. coli. Normal controls demonstrate a logarithmic normal distribution of I-AP activity in feces. The standard deviation of the intra- as well as of the inter-assay variation is 11.7%. This method represents a simple quantitative non-invasive in vivo assay for brush border damage under experimental and/or clinical conditions.
描述了一种使用抗人肠碱性磷酸酶（I-AP）IgG来测定粪便中人类肠碱性磷酸酶（I-AP）的免疫沉淀方法。用人胎盘碱性磷酸酶吸收后，抗人I-AP抗体具有单特异性，且与人肝碱性磷酸酶或大肠杆菌碱性磷酸酶无交叉反应。正常对照显示粪便中I-AP活性呈对数正态分布。批内和批间变异的标准差均为11.7%。该方法是一种用于在实验和/或临床条件下检测刷状缘损伤的简单、定量、非侵入性体内检测方法。
