Jones B C, Radley J M, Bradley T R, Hodgson G S
Exp Hematol. 1980 Jan;8(1):61-4.
The effect of 5-fluorouracil treatment of donor mice on the capacity of transplanted bone marrow to produce megakaryocytes in the spleens of lethally irradiated recipients has been examined. At both 10 and 13 days after transplantation, the spleens of recipients of 5-fluorouracil treated bone marrow had significantly more megakarocytes per unit area of spleen section than recipients injected with an equivalent number of spleen colony forming units from normal bone marrow. It is suggested that such treatment may provide a sensitive in vivo system for the investigation of endogenous factors influencing megakaryocyte progenitor proliferation. The results are consistent with the concept of stem cells being heterogeneous with respect to self-renewal capacity.
已研究了用5-氟尿嘧啶处理供体小鼠对移植骨髓在受致死性照射受体脾脏中产生巨核细胞能力的影响。在移植后10天和13天，接受5-氟尿嘧啶处理骨髓的受体脾脏每单位脾切片面积中的巨核细胞显著多于注射等量来自正常骨髓的脾集落形成单位的受体。提示这种处理可能为研究影响巨核细胞祖细胞增殖的内源性因素提供一个敏感的体内系统。这些结果与干细胞在自我更新能力方面具有异质性的概念一致。
