Burleigh B D, Reich E, Strickland S
Mol Cell Endocrinol. 1980 Aug;19(2):183-96. doi: 10.1016/0303-7207(80)90021-0.
This paper describes a method for obtaining cultures of rat ventral prostate epithelial cells. The prostate is first perfused with a collagenase solution before removal from the animal; subsequent mincing and incubation in vitro produces a suspension of alveolar cell clumps. Upon incubation, these clumps attach to the surface of the culture dish and spread into discrete epithelial cell colonies, which both retain differentiated morphology, and secrete a species of plasminogen activator that is characteristic of prostatic tissue. These properties were not observed in cultures prepared from single cell suspensions of the same organ. Maintenance of epithelial colony integrity and secretory activity specifically required the continued presence of stromal cells, glucocorticoids and insulin. Androgenic steroids were much less effective than glucocorticoids in stimulating plasminogen activator secretion and in maintaining colony integrity, in spite of the well-established androgen dependence of prostatic tissue morphology in vivo and in organ culture. Furthermore, no effects of prolactin were observed, either when this hormone was tested alone or in conjunction with steroid hormones. Of 3 retinoids tested, retinal was highly cytotoxic at concentrations in the range of 1 microM, whereas retinol and retinoic acid were without detectable effect.
本文描述了一种获取大鼠腹侧前列腺上皮细胞培养物的方法。在从动物体内取出前列腺之前，先用胶原酶溶液对其进行灌注；随后将其切碎并在体外培养，得到肺泡细胞团块的悬浮液。培养时，这些团块附着在培养皿表面，并扩散形成离散的上皮细胞集落，这些集落既保留了分化的形态，又分泌一种前列腺组织特有的纤溶酶原激活物。在由同一器官的单细胞悬液制备的培养物中未观察到这些特性。上皮细胞集落完整性和分泌活性的维持特别需要基质细胞、糖皮质激素和胰岛素的持续存在。尽管前列腺组织形态在体内和器官培养中已确定依赖雄激素，但雄激素类固醇在刺激纤溶酶原激活物分泌和维持集落完整性方面比糖皮质激素的效果要差得多。此外，无论是单独测试催乳素还是将其与类固醇激素联合测试，均未观察到其有任何作用。在所测试的3种类维生素A中，视黄醛在浓度为1微摩尔时具有高度细胞毒性，而视黄醇和视黄酸则未检测到有任何作用。
