Pfeifle B, Ditschuneit H H, Ditschuneit H
Horm Metab Res. 1980 Aug;12(8):381-5. doi: 10.1055/s-2007-996297.
Smooth muscle cells were grown from thoracic aortas of rats. The effect of insulin on the proliferation of these cells was studied by comparing the growth of cells in culture medium containing insulin and 1% fetal calf serum with growth of cells in culture medium containing only 1% serum and in culture medium containing 10% serum. Insulin in concentrations of 10, 50, 100, 1000 and 10 000 microunits/ml induced smooth muscle cells to stationary growth more rapidly than basal medium. This could be demonstrated as well in the logarithmic growth as in confluent cells grown in medium with 1% serum. However, the highest concentration of insulin did not stimulate growth to the same degree as medium containing 10% serum. Cells that were older in culture life (11th passage) did not show a growth response to insulin.
从大鼠胸主动脉中培养出平滑肌细胞。通过比较含胰岛素和1%胎牛血清的培养基中细胞的生长情况，与仅含1%血清的培养基以及含10%血清的培养基中细胞的生长情况，研究了胰岛素对这些细胞增殖的影响。浓度为10、50、100、1000和10000微单位/毫升的胰岛素比基础培养基能更迅速地诱导平滑肌细胞进入静止生长状态。这在对数生长期以及在含1%血清的培养基中生长的汇合细胞中都能得到证明。然而，胰岛素的最高浓度对生长的刺激程度不如含10%血清的培养基。培养代数较老（第11代）的细胞对胰岛素没有生长反应。
