Pechan W, Novick A C, Stowe N, Magnusson M, Straffon R A
Invest Urol. 1980 Jul;18(1):74-6.
We evaluated the effect of immunologic donor pretreatment followed by perfusion preservation on canine renal allograft survival. Donor-recipient pairs were selected on the basis of dog erythrocyte identity, a negative white blood-cell crossmatch, equivalent dog leukocyte matching, and significant mixed lymphocyte culture stimulation. Four hours before nephrectomy, all donors were pretreated with either normal saline or cyclophosphamide and methylprednisolone. After donor nephrectomy, all grafts were preserved by pulsatile perfusion for 24 hr. Transplantation into the matched recipient was performed with simultaneous bilateral nephrectomy. No significant difference was found in the postoperative serum creatinine levels or median allograft survival between the two groups. This controlled study showed no beneficial effect of donor pretreatment on survival of perfused canine renal allografts.
我们评估了免疫供体预处理后进行灌注保存对犬肾同种异体移植存活的影响。供体-受体对是根据犬红细胞同一性、阴性白细胞交叉配型、等效犬白细胞配型以及显著的混合淋巴细胞培养刺激来选择的。在肾切除术4小时前，所有供体均用生理盐水或环磷酰胺和甲基强的松龙进行预处理。供体肾切除术后，所有移植物通过搏动灌注保存24小时。在同时进行双侧肾切除术的情况下将移植物移植到匹配的受体中。两组之间术后血清肌酐水平或同种异体移植中位存活时间均未发现显著差异。这项对照研究表明，供体预处理对灌注保存的犬肾同种异体移植的存活没有有益影响。
