Scher I, Berning A K, Kessler S, Finkelman F D
J Immunol. 1980 Oct;125(4):1686-93.
Affinity-purified anti-mouse mu and delta antibodies and flow microfluorometric techniques were utilized to study the frequency and distribution of sIgM and sIgD on adult and neonatal B lymphocytes of immunologically normal mice and mice with the immune-defect characteristic of the CBA/N strain. Neonatal normal B lymphocytes can be distinguished from their adult counterparts by the presence of increased amounts of sIgM on sIgM+ cells and by the increased number of sIgM+IgD- cells. The most primitive B lymphocytes of immunologically normal mice appear to reside in the bone marrow of 1- to 2-wk-old mice and are distinguished from splenic B lymphocytes at this time by their relatively low amounts of sIgM. However, in the adult, splenic and bone marrow B lymphocytes are indistinguishable with regard to the quantities of their sIgM or sIgD. Adult immune-defective mice have increased amounts of sIgM on their B lymphocytes, although the number of sIgM+ IgD- cells in these mice is not increased. During development, however, larger than normal numbers of sIgM+ IgD- cells are found in the spleen and bone marrow of these mice; and the low frequency of such cells seen in normal adults is not achieved in immune defectives until after the 56th day of life. Thereafter, immune-defective mice have low frequencies of B lymphocytes with high relative amounts of sIgM, even after 1 yr or age.
利用亲和纯化的抗小鼠μ链和δ链抗体以及流式微量荧光技术，研究了免疫正常小鼠和具有CBA/N品系免疫缺陷特征的小鼠的成年和新生B淋巴细胞上sIgM和sIgD的频率和分布。新生正常B淋巴细胞可通过sIgM+细胞上sIgM含量增加以及sIgM+IgD-细胞数量增加与成年B淋巴细胞区分开来。免疫正常小鼠最原始的B淋巴细胞似乎存在于1至2周龄小鼠的骨髓中，此时它们与脾脏B淋巴细胞的区别在于sIgM含量相对较低。然而，在成年小鼠中，脾脏和骨髓B淋巴细胞的sIgM或sIgD数量没有区别。成年免疫缺陷小鼠的B淋巴细胞上sIgM含量增加，尽管这些小鼠中sIgM+IgD-细胞的数量没有增加。然而，在发育过程中，这些小鼠的脾脏和骨髓中发现的sIgM+IgD-细胞数量比正常小鼠多；直到出生后第56天，免疫缺陷小鼠才达到正常成年小鼠中此类细胞的低频率。此后，即使在1岁或更大年龄后，免疫缺陷小鼠中具有高相对含量sIgM的B淋巴细胞频率也较低。
