Murer H, Kinne R
J Membr Biol. 1980 Jul 15;55(2):81-95. doi: 10.1007/BF01871151.
Epithelia are multicompartment and multicomponent systems performing transcellular and paracellular transport in a very complex manner. One way to get a deeper understanding of the function of such a complex system is to dissect it into the single components and then, after having defined the components under well-controlled conditions, to try to describe the behavior of the whole system on the basis of the properties of the single components. This article deals with the analysis of isolated plasma membranes derived from the luminal and contraluminal face of epithelial cells, predominantly renal proximal tubular and small intestinal cells. It is aimed to give an overview of methods used to isolate and separate plasma membranes, to study their transport properties as membrane vesicles, and also to address the question of how information gained with the isolated membranes corresponds to observations made in the intact cell using other, notably electrophysiological, measurements. The review also critically evaluates the limitations of the approach and thereby tries to set the work on isolated membranes in the proper perspective within the field of transport physiology.
上皮组织是多隔室和多组分系统，以非常复杂的方式进行跨细胞和细胞旁运输。深入理解这样一个复杂系统功能的一种方法是将其分解为单个组分，然后在严格控制的条件下定义这些组分后，根据单个组分的特性来描述整个系统的行为。本文论述了源自上皮细胞管腔面和对管腔面（主要是肾近端小管细胞和小肠细胞）的分离质膜的分析。目的是概述用于分离和分选质膜的方法，研究其作为膜泡的运输特性，同时探讨从分离膜获得的信息如何与使用其他测量方法（尤其是电生理学测量）在完整细胞中所做的观察结果相对应。本综述还批判性地评估了该方法的局限性，从而试图将分离膜的研究工作在运输生理学领域中置于恰当的视角。
