De Potter W P, Fraeyman N H, Plum J, De Schaepdryver A F
Tissue Cell. 1980;12(2):227-32. doi: 10.1016/0040-8166(80)90001-4.
Dopamine-beta-hydroxylase (D beta H), a glycoprotein enzyme which converts dopamine into noradrenaline, was purified from C1300 mouse neuroblastoma and used to raise antibodies in rabbits. Using an indirect immunofluorescence technique the cellular localization of D beta H in C1300 mouse neuroblastoma was compared with that of the superior cervical ganglion. C1300 neuroblastoma D beta H was found to be predominantly localized in the plasma membrane, in contrast to its intracellular localization in the superior cervical ganglion of A/J mice. At least part of the enzyme was found to be associated with the external side of the plasma membrane.
多巴胺-β-羟化酶（DβH）是一种将多巴胺转化为去甲肾上腺素的糖蛋白酶，从C1300小鼠神经母细胞瘤中纯化出来，并用于在兔体内产生抗体。使用间接免疫荧光技术，将C1300小鼠神经母细胞瘤中DβH的细胞定位与颈上神经节的细胞定位进行了比较。发现C1300神经母细胞瘤DβH主要定位于质膜，这与其在A/J小鼠颈上神经节中的细胞内定位形成对比。发现至少部分该酶与质膜的外侧相关联。
