Khaitova N M, Il'ina G S, Liapunova N A
Tsitologiia. 1980 Jun;22(6):640-5.
By the autoradiography, the increase of the mean rate of replication fork movement in DNA was shown for human skin fibroblasts during S-period induced by reseeding the stationary culture; the portion of high-rate replicons growing parallel to the increase in numbers of cells, in which the constitutive heterochromatin was replicating. Furthermore, the heterogeneity of replicons was investigated in regard to the rate of DNA chain growth in asynchronous cell populations of human and three rodent species (Microtus agrestis, Tscherskia triton, Cricetulus griseus). A positive correlation was found between the portion of replicons, with a relatively high rate of replication (more than 60 micron/h), and the relative amounts of C-heterochromatin in genomes of these species. On the basis of these studies it has been assumed, that the replicons with the high rate of DNA chain growth belong to the constitutive heterochromatin.
通过放射自显影法表明，对于静止培养后重新接种的人皮肤成纤维细胞，在S期DNA中复制叉移动的平均速率增加；高速率复制子的比例与细胞数量增加平行增长，其中组成型异染色质正在复制。此外，还研究了人类和三种啮齿动物（田鼠、东方田鼠、灰仓鼠）异步细胞群体中复制子在DNA链生长速率方面的异质性。发现复制速率相对较高（超过60微米/小时）的复制子比例与这些物种基因组中C-异染色质的相对含量之间存在正相关。基于这些研究，人们假定DNA链生长速率高的复制子属于组成型异染色质。
