Macromolecular derivatives of NAD and their application.

The effects of the properties of polymer chain and NAD density on the cofactor activity of polymeric NAD derivatives were investigated. NAD-N6- [N-(N-acryloyl-l-methoxycarbonyl-5-aminopentyl)-propioamide] (monomeric NAD derivative) was copolymerized with different kinds of vinyl monomers. The resulting polymeric NAD derivatives, except for that prepared with methacrylamide, had similar cofactor activities. These results show that the size and charge of the vinyl monomers do not have a great effect on the cofactor activity of polymeric NAD derivatives. The polymeric NAD derivative prepared by copolymerization with methacrylamide had the highest cofactor activities, and the activities for lactate and yeast alcohol dehydrogenases decreased with an increase in the NAD density of the polymer. These NAD density effects were mainly due to the inhibition by the polymeric NAD derivative with a high NAD density.