在BHK 21细胞中，肌动蛋白与吞噬体短暂关联的免疫荧光证据。

Immunofluorescent evidence for a transient association of actin with phagosomes in BHK 21 cells.

作者信息

Storrie B, Chadwick R T

出版信息

Cell Biol Int Rep. 1980 Sep;4(9):873-9. doi: 10.1016/0309-1651(80)90186-1.

Abstract

The association of actin with latex phagosomes in BHK 21 cells, a normal fibroblastic cell line, was studied using fluoescent antibody to anti-DNase I. DNase I binds specifically to actin and in fixed, acetone-extracted BHK cells much of the immunofluorescent staining is restricted to microfilament bundles. A concentrated rim of actin specific fluorescence is observed about newly formed phagosomes. After long ingestion periods of any actin specific fluorescence. The data indicate that actin polymerization about phagosomes in fibroblasts is a transient event persisting for approximately 2 min.

摘要

利用抗脱氧核糖核酸酶I的荧光抗体，研究了肌动蛋白与正常成纤维细胞系BHK 21细胞中乳胶吞噬体的关联。脱氧核糖核酸酶I特异性结合肌动蛋白，在固定的、经丙酮提取的BHK细胞中，许多免疫荧光染色局限于微丝束。在新形成的吞噬体周围观察到肌动蛋白特异性荧光的浓集边缘。在长时间摄取后，任何肌动蛋白特异性荧光均消失。数据表明，成纤维细胞中吞噬体周围的肌动蛋白聚合是一个持续约2分钟的短暂事件。