Morimoto K, Matsunaga M, Hara A, Kawai C
Hypertension. 1980 Sep-Oct;2(5):680-85. doi: 10.1161/01.hyp.2.5.680.
The mechanism of activation of inactive renin was studied in normal human plasma. The molecular weight of active renin and those of inactive renin before and after activation were analyzed by sephadex gel filtration. Active renin of human plasma had a molecular weight of 48,000 +/- 1000. Trypsin treatment and cold treatment activated inactive renin of a molecular weight of 54,000 +/- 1000. The inactive renin apparently did not change its molecular weight after activation. "Cryoactivation" of inactive renin was possible only when whole plasma was used. When the whole plasma was fractionated by gel filtration, cryoactivation was not observed in any of the fractions. Cryoactivation requires certain plasma factor(s) contained in some fractions. Plasma kallikrein is likely to be a major factor required for the cryoactivation of inactive renin, whereas some other factors may also participate in this mechanism.
在正常人血浆中研究了无活性肾素的激活机制。通过葡聚糖凝胶过滤分析了活性肾素以及激活前后无活性肾素的分子量。人血浆中的活性肾素分子量为48,000±1000。胰蛋白酶处理和冷处理可激活分子量为54,000±1000的无活性肾素。无活性肾素激活后其分子量显然没有变化。无活性肾素的“冷激活”仅在使用全血浆时才有可能。当通过凝胶过滤对全血浆进行分级分离时，在任何级分中均未观察到冷激活。冷激活需要某些级分中所含的特定血浆因子。血浆激肽释放酶可能是无活性肾素冷激活所需的主要因子，而其他一些因子也可能参与这一机制。
