Keilmann F, Böhme D, Santo L
Appl Environ Microbiol. 1980 Sep;40(3):458-61. doi: 10.1128/aem.40.3.458-461.1980.
We describe an instrument for monitoring either transmitted or scattered light intensity, or both, simultaneously on up to eight channels. The use of a laser light source (at 632.8-nm wavelength) provides high accuracy and dynamic range: optical density can be measured from 0.0004 up to 6, and a scattered light fraction down to 10(-6) can be resolved. Built-in thermostat and magnetic stirrers allow precise monitoring of aqueous microbial growth over a practical range of 4 orders of magnitude of cell concentration.
我们描述了一种仪器,可同时在多达八个通道上监测透射光强度或散射光强度,或两者兼而有之。使用激光光源(波长为632.8纳米)可提供高精度和动态范围:光密度可从0.0004测量到6,并且可分辨低至10^(-6)的散射光分数。内置恒温器和磁力搅拌器可在实际的4个数量级细胞浓度范围内精确监测水性微生物的生长。