Dyck R F, Lockwood C M, Kershaw M, McHugh N, Duance V C, Baltz M L, Pepys M B
J Exp Med. 1980 Nov 1;152(5):1162-74. doi: 10.1084/jem.152.5.1162.
Glomerular and other vascular basement membranes were found to contain an antigen that was immunochemically indistinguishable from serum amyloid P-component. There was no immunological cross-reactivity between antisera to serum amyloid P-component and to collagen types I, III, IV, or V. The amyloid P-component antigen was confined to the endothelial aspect, the lamina rara interna, of glomerular basement membrane. It could not be eluted by high-ionic-strength saline, EDTA, dithiothreitol, or either polar or nonpolar detergents, but was released into solution when isolated glomerular basement membrane was digested by highly purified bacterial collagenase. Most of these P-component molecules and their constituent polypeptide chains were of higher molecular weight and lower isoelectric point than serum amyloid P-component. These findings indicate that, as well as being a normal plasma protein and a universal constituent of amyloid deposits, P-component is also a normal matrix glycoprotein of basement membrane in which it is covalently linked to collagen and/or other matrix proteins. This may be relevant both to the pathogenesis of amyloidosis and to other aspects of physiology and pathology of basement membranes.
肾小球及其他血管基底膜被发现含有一种抗原，该抗原在免疫化学上与血清淀粉样P成分无法区分。血清淀粉样P成分抗血清与I、III、IV或V型胶原之间不存在免疫交叉反应。淀粉样P成分抗原局限于肾小球基底膜的内皮侧，即内疏松层。它不能被高离子强度盐水、乙二胺四乙酸（EDTA）、二硫苏糖醇或极性或非极性去污剂洗脱，但当分离的肾小球基底膜被高度纯化的细菌胶原酶消化时，会释放到溶液中。与血清淀粉样P成分相比，这些P成分分子及其组成的多肽链大多具有更高的分子量和更低的等电点。这些发现表明，P成分不仅是一种正常的血浆蛋白和淀粉样沉积物的普遍成分，也是基底膜的一种正常基质糖蛋白，其中它与胶原和/或其他基质蛋白共价连接。这可能与淀粉样变性的发病机制以及基底膜生理和病理的其他方面都有关。
