叠氮多核苷酸作为光亲和试剂。
Azidopolynucleotides as photoaffinity reagents.
作者信息
Cartwright I L, Hutchinson D W
出版信息
Nucleic Acids Res. 1980 Apr 11;8(7):1675-91. doi: 10.1093/nar/8.7.1675.
Abstract
Polynucleotides containing adenosine and 8-azidoadenosine or inosine and 8-azidoinosine residues have been prepared from mixtures of nucleoside diphosphates using polynucleotide phosphorylase from Escherichia coli. These copolymers can form complexes with polyuridylic or polycytidylic acids respectively. Single stranded poly(adenylic, 8-azidoadenylic acid) [poly(A,z8A)] has been used as a photoaffinity reagent to explore the subunit topography of RNA polymerase from E. coli.
摘要
利用来自大肠杆菌的多核苷酸磷酸化酶，从核苷二磷酸混合物中制备了含有腺苷和8-叠氮腺苷残基或肌苷和8-叠氮肌苷残基的多核苷酸。这些共聚物可分别与聚尿苷酸或聚胞苷酸形成复合物。单链聚（腺苷酸、8-叠氮腺苷酸）[聚（A，z8A）]已被用作光亲和试剂，以探索大肠杆菌RNA聚合酶的亚基拓扑结构。
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