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叠氮多核苷酸作为光亲和试剂。

Azidopolynucleotides as photoaffinity reagents.

作者信息

Cartwright I L, Hutchinson D W

出版信息

Nucleic Acids Res. 1980 Apr 11;8(7):1675-91. doi: 10.1093/nar/8.7.1675.

DOI:10.1093/nar/8.7.1675
PMID:7001370
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324024/
Abstract

Polynucleotides containing adenosine and 8-azidoadenosine or inosine and 8-azidoinosine residues have been prepared from mixtures of nucleoside diphosphates using polynucleotide phosphorylase from Escherichia coli. These copolymers can form complexes with polyuridylic or polycytidylic acids respectively. Single stranded poly(adenylic, 8-azidoadenylic acid) [poly(A,z8A)] has been used as a photoaffinity reagent to explore the subunit topography of RNA polymerase from E. coli.

摘要

利用来自大肠杆菌的多核苷酸磷酸化酶，从核苷二磷酸混合物中制备了含有腺苷和8-叠氮腺苷残基或肌苷和8-叠氮肌苷残基的多核苷酸。这些共聚物可分别与聚尿苷酸或聚胞苷酸形成复合物。单链聚（腺苷酸、8-叠氮腺苷酸）[聚（A，z8A）]已被用作光亲和试剂，以探索大肠杆菌RNA聚合酶的亚基拓扑结构。

相似文献

1
Azidopolynucleotides as photoaffinity reagents.叠氮多核苷酸作为光亲和试剂。
Nucleic Acids Res. 1980 Apr 11;8(7):1675-91. doi: 10.1093/nar/8.7.1675.
2
Mapping of a contact for the RNA 3' terminus in the largest subunit of RNA polymerase.
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3
Photoaffinity labeling of DNA-dependent RNA polymerase from Escherichia coli with 8-azidoadenosine 5'-triphosphate.用8-叠氮腺苷5'-三磷酸对大肠杆菌依赖DNA的RNA聚合酶进行光亲和标记。
Biochemistry. 1984 Jun 19;23(13):2843-8. doi: 10.1021/bi00308a001.
4
Covalent modification of the inhibitor binding site(s) of Escherichia coli ADP-glucose synthetase: specific incorporation of the photoaffinity analogue 8-azidoadenosine 5'-monophosphate.
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5
Extensive synthesis of poly[r(G-C)] using Escherichia coli RNA polymerase.
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6
Detection of nucleoside triphosphate binding sites of two types in Escherichia coli RNA-polymerase by affinity labeling.通过亲和标记检测大肠杆菌RNA聚合酶中两种类型的核苷三磷酸结合位点。
Mol Biol Rep. 1980 Mar 31;6(1):31-4. doi: 10.1007/BF00775751.
7
Properties of polynucleotide phosphorylase from E. coli Polynucleotide phosphorylase-deficient and thermo-sensitive mutants.
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本文引用的文献

1
THE ENZYMATIC SYNTHESIS OF RIBONUCLEIC ACID. V. THE INTERACTION OF RIBONUCLEIC ACID POLYMERASE WITH NUCLEIC ACIDS.核糖核酸的酶促合成。V.核糖核酸聚合酶与核酸的相互作用。
J Biol Chem. 1965 May;240:2101-9.
2
INFLUENCE OF DNA SECONDARY STRUCTURE ON DNA-DEPENDENT POLYPEPTIDE SYNTHESIS.DNA二级结构对依赖DNA的多肽合成的影响。
J Mol Biol. 1964 Aug;9:452-71. doi: 10.1016/s0022-2836(64)80219-9.
3
ENZYMATIC SYNTHESIS OF RIBONUCLEIC ACID. III. THE RIBONUCLEIC ACID-PRIMED SYNTHESIS OF RIBONUCLEIC ACID WITH MICROCOCCUS LYSODEIKTICUS RIBONUCLEIC ACID POLYMERASE.核糖核酸的酶促合成。III. 用溶壁微球菌核糖核酸聚合酶进行核糖核酸引发的核糖核酸合成
J Biol Chem. 1964 Jan;239:186-95.
4
Inhibition of RNA polymerase by RNA.RNA对RNA聚合酶的抑制作用。
J Mol Biol. 1963 Jul;7:100-3. doi: 10.1016/s0022-2836(63)80024-8.
5
Studies on the formation of two- and three-stranded polyribonucleotides.关于双链和三链多核糖核苷酸形成的研究。
Biochim Biophys Acta. 1957 Dec;26(3):457-68. doi: 10.1016/0006-3002(57)90091-4.
6
Template properties of polyribonucleotides containing uracil or modified uracil in the RNA polymerase reaction.
J Mol Biol. 1967 Feb 14;23(3):417-39. doi: 10.1016/s0022-2836(67)80116-5.
7
Studies on the binding of RNA polymerase to polynucleotides.RNA聚合酶与多核苷酸结合的研究。
J Mol Biol. 1966 Dec 28;22(2):199-209. doi: 10.1016/0022-2836(66)90126-4.
8
Polynucleotides. VII. Synthesis of ribopolynucleotides containing 8-substituted purine nucleotides by polynucleotide phosphorylase.多核苷酸。VII. 利用多核苷酸磷酸化酶合成含8-取代嘌呤核苷酸的核糖多核苷酸。
Biochemistry. 1969 Feb;8(2):736-43. doi: 10.1021/bi00830a040.
9
Polynucleotides. 8. A spectral approach to the equilibria between polyriboadenylate and polyribouridylate and their complexes.多核苷酸。8. 一种研究聚腺苷酸与聚尿苷酸及其复合物之间平衡的光谱学方法。
J Mol Biol. 1967 Dec 14;30(2):291-308.
10
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.