Date T, Goodman J M, Wickner W T
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4669-73. doi: 10.1073/pnas.77.8.4669.
The coat protein of coliphage M13 spans the host cell cytoplasmic membrane prior to its assembly into extruding virus. It is made as a soluble cytoplasmic precursor, termed "procoat," with 23 extra amino acid residues at the NH2 terminus. Procoat binds to the cell membrane and is converted proteolytically to coat protein. When the electrochemical gradient of an infected cell is rapidly dissipated by uncouplers, procoat still binds to the plasma membrane but is not converted to coat. We report here that membrane-bound procoat is only detected at the inner face of the cytoplasmic membrane and that uncouplers prevent it from integrating into a transmembrane conformation.
大肠杆菌噬菌体M13的外壳蛋白在组装成出芽病毒之前跨越宿主细胞质膜。它最初作为一种可溶性细胞质前体产生，称为“前衣壳”，在NH2末端有23个额外的氨基酸残基。前衣壳与细胞膜结合，并通过蛋白水解作用转化为外壳蛋白。当感染细胞的电化学梯度被解偶联剂迅速消散时，前衣壳仍与质膜结合，但不会转化为外壳蛋白。我们在此报告，膜结合的前衣壳仅在细胞质膜的内表面被检测到，并且解偶联剂阻止它整合到跨膜构象中。
