Ikonomov L
Vet Med Nauki. 1980;17(2):79-84.
The study concerned the formation of gas and acid in broth with lactose and glucose and brilliant green at 30,37 and 44 degrees C and of indole in peptone water at 30, 37 and 44 degrees C by 842 coliform strains isolated from 386 samples of dairy products (E. coli I--496, E. coli--51, Citrob. freundi--87, Citrob. intermedium--46, Enterobacter aerogenes--98, Enterobacter cloacae -- 32 and K1. aerogenes 32). It was found that Eijkman's test (formation of gas and acid from lactose in broth with brilliant green at 44 degrees C) yields no real results and cannot be used for rapid differentiation of the fecal coliforms. The test of McKenzie, Taylor and Gilbert (formation of gas and acid from lactose in broth with brilliant green at 44 degrees C and indole at 44 degrees C) gives satisfactory results for quick determination of the expected E. coli I amount. E. coli I amount should be determined solely on the basis of IMVIC tests.
该研究关注了从386份乳制品样本中分离出的842株大肠菌群菌株(大肠杆菌I型496株、大肠杆菌51株、弗氏柠檬酸杆菌87株、中间柠檬酸杆菌46株、产气肠杆菌98株、阴沟肠杆菌32株和气态克雷伯菌32株)在30℃、37℃和44℃下于含乳糖、葡萄糖和亮绿的肉汤中产生气体和酸的情况,以及在30℃、37℃和44℃下于蛋白胨水中产生吲哚的情况。研究发现,艾克曼试验(44℃下在含亮绿的肉汤中由乳糖产生气体和酸)无法得出实际结果,不能用于快速区分粪大肠菌群。麦肯齐、泰勒和吉尔伯特试验(44℃下在含亮绿的肉汤中由乳糖产生气体和酸以及44℃下产生吲哚)对于快速测定预期的大肠杆菌I型数量给出了满意结果。大肠杆菌I型数量应仅基于IMVIC试验来确定。