Bindschedler O, De Man J C, Curiat G
Zentralbl Bakteriol Naturwiss. 1981;136(2):146-51. doi: 10.1016/s0323-6056(81)80036-5.
Five culture media have been evaluated for the detection of coliforms, E. coli, and Enterobacteriaceae in several varieties of dairy products (fresh and dehydrated) and dehydrated cocoa products. Those media were: Brilliant green bile, Lauryl sulfate tryptone, Lactose glutamic acid, Enteric Enrichment broth, and Lactose broth. The medium with the highest sensitivity to coliforms detection was Lactose glutamic acid broth. Several times Brilliant green bile, Lactose or Enteric Enrichment broth were significantly less sensitive than the other two media. Except once, when it was significantly better, Lauryl sulfate broth was not significantly different from the other media. False positive results were most frequently found during the examination of dairy products with Lactose glutamic acid and Lactose broth, consequently confirmation reactions have to be carried out when these media are used. Frequently, Enteric Enrichment broth was less satisfactory for the detection of Enterobacteriaceae than the other media used. All five media showed more coliforms after 48 h than after 24 h of incubation at 30 degrees C. Whenever a significant difference existed in the sensitivity to determine E. coli, Lactose glutamic acid broth was significantly better than the other media.
已对五种培养基进行评估，以检测几种乳制品（新鲜和脱水）及脱水可可制品中的大肠菌群、大肠杆菌和肠杆菌科细菌。这些培养基分别是：煌绿胆盐培养基、月桂基硫酸盐胰蛋白胨培养基、乳糖谷氨酸培养基、肠道增菌肉汤和乳糖肉汤。对大肠菌群检测灵敏度最高的培养基是乳糖谷氨酸肉汤。煌绿胆盐培养基、乳糖或肠道增菌肉汤的灵敏度多次显著低于其他两种培养基。除了有一次显著更好外，月桂基硫酸盐肉汤与其他培养基无显著差异。在用乳糖谷氨酸培养基和乳糖肉汤检测乳制品时，最常出现假阳性结果，因此使用这些培养基时必须进行确认反应。通常，肠道增菌肉汤对肠杆菌科细菌的检测效果不如其他所用培养基。在30摄氏度下培养48小时后，所有五种培养基中的大肠菌群数量均比培养24小时后更多。每当在检测大肠杆菌的灵敏度上存在显著差异时，乳糖谷氨酸肉汤均显著优于其他培养基。
