Clarkson P M, Katch F I, Kroll W, Lane R, Kamen G
Am J Clin Nutr. 1980 Nov;33(11):2245-52. doi: 10.1093/ajcn/33.11.2245.
Regional adipose cell size was assessed in 18 males ranging in age from 20 to 36 years. These measures were correlated to total body fat determined by hydrostatic weighing. Adipose samples were removed from the gluteal, abdominal, and subscapular regions using a needle aspiration technique. The tissue was incubated in collagenase to release individual cells that were immediately photographed under a microscope. To establish the reliability of adipose cell size assessment over time, fat biopsies were secured on 2 separate days. No significant difference was found for any region between day 1 and 2 adipose cell measures. Gluteal cell diameter (90.3 mu) was significantly larger than the abdominal (81.0 mu) and subscapular (78.6 mu) cell diameter (90.3 mu) was significantly larger than the abdominal (81.0 mu) and subscapular (78.6 mu) cell diameters. Total body fat correlated highest with gluteal cell size (r = 0.76) compared with the abdominal (r = 0.67) or subscapular (r = 0.70) regions. This study also examined the number of adipose cells required to subscapular (r = 0.70) regions. This study also examined the number of adipose cells required to obtain a reliable and representative mean value of adipose cell size. Using a sequential estimation analysis it was found that adipose cell diameters of the abdominal, gluteal, or subscapular regions can be reliably estimated with fewer than 100 cells.
对18名年龄在20至36岁之间的男性进行了局部脂肪细胞大小评估。这些测量结果与通过水下称重法测定的全身脂肪相关。使用针吸技术从臀、腹部和肩胛下区域采集脂肪样本。将组织在胶原酶中孵育以释放单个细胞，这些细胞立即在显微镜下拍照。为了确定脂肪细胞大小评估随时间的可靠性，在两个不同的日子采集了脂肪活检样本。第1天和第2天的脂肪细胞测量结果在任何区域均未发现显著差异。臀细胞直径（90.3微米）明显大于腹部（81.0微米）和肩胛下（78.6微米）细胞直径。与腹部（r = 0.67）或肩胛下（r = 0.70）区域相比，全身脂肪与臀细胞大小的相关性最高（r = 0.76）。该研究还检查了获得可靠且具有代表性的脂肪细胞大小平均值所需的脂肪细胞数量。使用序贯估计分析发现，腹部、臀部或肩胛下区域的脂肪细胞直径用少于100个细胞即可可靠估计。
