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采用极限稀释分析法对液体微量培养中的造血祖细胞（集落形成单位 - 粒细胞和爆式红系集落形成单位）进行计数。

Enumeration of haemopoietic progenitors (CFU-C and BFU-E) in liquid microculture using a limiting dilution analysis.

作者信息

Marchal G, Milon G

出版信息

Ann Immunol (Paris). 1980 Jul-Aug;131D(1):111-5.

PMID:7002016
Abstract

In order to overcome the difficulty of scoring multicentric colonies of haemopoietic progenitor cells, cultures in 10 microliter of liquid medium were grown. Plated at three different cell concentrations, progenitor cells (CFU-C or BFU-E) are not present in all wells. Their presence is easily scored in each well of microculture 7 days after incubation. A limiting dilution analysis allows one to obtain accurate quantification.

摘要

为了克服对造血祖细胞多中心集落进行评分的困难，在10微升液体培养基中进行培养。以三种不同的细胞浓度接种，并非所有孔中都存在祖细胞（CFU-C或BFU-E）。孵育7天后，在微量培养的每个孔中很容易对它们的存在进行评分。有限稀释分析可实现准确的定量。

相似文献

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Ann Immunol (Paris). 1980 Jul-Aug;131D(1):111-5.
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