da Costa M, Sharon M
Br J Haematol. 1980 Dec;46(4):575-9. doi: 10.1111/j.1365-2141.1980.tb06014.x.
The blast transformation of human lymphocytes exposed to phytohaemagglutinin was associated with an increase in the intracellular concentration of a folate-binding protein. The protein was measured by a radioimmunoassay and by immunofluorescent microscopy using an antiserum raised against purified folate-binding protein prepared from chronic myelogenous leukaemia cells. Because it has been previously shown that protein-bound folate cannot function as a cofactor or substrate, the synthesis of this binder during some phase of the replicative cycle may serve to modulate folate-dependent RNA and DNA synthesis by controlling the concentration of free folate.
暴露于植物血凝素的人淋巴细胞的原始细胞转化与一种叶酸结合蛋白的细胞内浓度增加有关。该蛋白通过放射免疫测定法以及使用针对从慢性粒细胞白血病细胞制备的纯化叶酸结合蛋白产生的抗血清的免疫荧光显微镜进行测量。因为先前已经表明蛋白质结合的叶酸不能作为辅因子或底物发挥作用，所以在复制周期的某个阶段这种结合物的合成可能通过控制游离叶酸的浓度来调节叶酸依赖性RNA和DNA的合成。
