Watanabe S, Kuroki M, Sato Y, Shimosato Y, Hasegawa T
Cancer. 1980 Dec 1;46(11):2438-45. doi: 10.1002/1097-0142(19801201)46:11<2438::aid-cncr2820461124>3.0.co;2-x.
A cell line (NH-AR strain) has been established in vitro from a patient with malignant lymphoma, nodular histiocytic type, and maintained in continuous growth for more than 18 months. The cells grew floating in the culture medium forming characteristic balls. The doubling time of the total cell population was 38.4 hours. Surface and cytoplasmic immunoglobulin synthesis were monoclonal gamma heavy chain and lambda light chain. Chromosome analysis of the cell line revealed 47/48,XY,+A with a mode of 47,XY,+1,1p-,t(2q-;3pt+),t(18q-;14q+), which was identical to that obtained from the biopsied lymph node. Ultrastructurally, tight interdigitation of the cytoplasmic processes was reminiscent of the labyrinthine structure of the nodular lymphoma cells. Its nodular growth pattern is discussed in relation to other lymphoblastoid cell lines.
已从一名结节性组织细胞型恶性淋巴瘤患者体内体外建立了一种细胞系（NH-AR株），并持续生长超过18个月。细胞在培养基中呈悬浮生长，形成特征性的球状物。总细胞群体的倍增时间为38.4小时。表面和细胞质免疫球蛋白合成呈单克隆γ重链和λ轻链。该细胞系的染色体分析显示为47/48，XY，+A，众数为47，XY，+1，1p-，t(2q-;3pt+)，t(18q-;14q+)，这与从活检淋巴结获得的结果相同。超微结构上，细胞质突起的紧密交错让人联想到结节性淋巴瘤细胞的迷宫样结构。结合其他淋巴母细胞系讨论了其结节状生长模式。
