Ley K D
J Cell Physiol. 1980 May;103(2):255-62. doi: 10.1002/jcp.1041030210.
Mitochondrial protein synthesis was measured in line CHO cells after phases of the cell cycle were synchronized by isoleucine deprivation or mitotic selection. Maximum incorporation of [3H] leucine into mitochondrial polypeptides occurred within 2 hours after isoleucine was added to initiate G1 traverse. In cells synchronized in G1 by mitotic selection, the rate of mitochondrial protein synthesis was fairly constant throughout the cell cycle. SDS-polyacrylamide gel electrophoretic profiles of labeled mitochondrial polypeptides were similar in cells synchronized by either isoleucine deprivation or mitotic selection. Obvious changes in the distribution of polypeptides were not detected during various phases of the cell cycle. The increased rate of incorporation of [3H] leucine into mitochondrial polypeptides after reversal of G1-arrest may indicate that mitochondrial protein synthesis and possibly mitochondrial biogenesis are synchronized in CHO cells deprived of isoleucine.
通过异亮氨酸剥夺或有丝分裂选择使细胞周期各阶段同步化后，测定了中国仓鼠卵巢（CHO）细胞系中的线粒体蛋白质合成。在添加异亮氨酸以启动G1期进程后的2小时内，[3H]亮氨酸在线粒体多肽中的掺入量达到最大值。在通过有丝分裂选择同步于G1期的细胞中，线粒体蛋白质合成速率在整个细胞周期中相当恒定。通过异亮氨酸剥夺或有丝分裂选择同步化的细胞中，标记的线粒体多肽的SDS-聚丙烯酰胺凝胶电泳图谱相似。在细胞周期的各个阶段未检测到多肽分布的明显变化。G1期阻滞解除后，[3H]亮氨酸在线粒体多肽中的掺入速率增加，这可能表明在缺乏异亮氨酸的CHO细胞中，线粒体蛋白质合成以及可能的线粒体生物发生是同步的。
