Kobayashi N, Allen N, Clendenon N R, Ko L W
J Neurosurg. 1980 Dec;53(6):808-15. doi: 10.3171/jns.1980.53.6.0808.
The widely used intracerebral tumor implantation method by freehand injection into parietal or hippocampal areas of the rat brain has proven inadequate for reliable experimental therapeutic studies. Problems include poor intracerebral growth yields and significant rates of spread to extracranial tissues, lungs, and spinal cord. Major variables have been examined experimentally on a model using nitrosourea-induced nervous system tumor cell lines in sygeneic rats. A rapid stereotaxic method greatly improved the consistency of tumor placement. The optimal site was found to be the caudate nucleus. The production of a spheroid intracerebral growth was further facilitated by the use of 1% agar in the cell suspension medium and by an injection volume of 10 mu1 containing at least 10(4) cells. Further improvements involved injection technique and flushing of the operative field. These modifications have resulted in a 99% to 100% yield of intracerebral growth, with a marked reduction in the number and size of extracranial extensions and with distant metastasis rates of 0% to 5%. These results have continually improved with further experience. The method is satisfactory for radiation and chemotherapeutic trials in which survival time as an index of tumor size may be used an an end point.
广泛应用的通过徒手注射将肿瘤植入大鼠脑顶叶或海马区的方法，已被证明不适用于可靠的实验性治疗研究。问题包括脑内生长产量低以及肿瘤显著扩散至颅外组织、肺部和脊髓。在同基因大鼠中使用亚硝基脲诱导的神经系统肿瘤细胞系的模型上，对主要变量进行了实验研究。一种快速立体定向方法极大地提高了肿瘤植入的一致性。发现最佳部位是尾状核。在细胞悬液培养基中使用1%的琼脂以及注射10微升至少含有10⁴个细胞的悬液，进一步促进了脑内球形生长的形成。进一步的改进涉及注射技术和术野冲洗。这些改进使脑内生长产量达到99%至100%，颅外扩展的数量和大小显著减少，远处转移率为0%至5%。随着经验的不断积累，这些结果持续得到改善。该方法对于以生存时间作为肿瘤大小指标的放疗和化疗试验来说是令人满意的，可将其作为终点指标。
