Livni N, Laufer A, Levo Y
J Pathol. 1980 Dec;132(4):343-8. doi: 10.1002/path.1711320405.
A specific rabbit anti-murine amyloid A protein (anti-AA) has been used in the immunoperoxidase (IP) technique for the detection of amyloid in formalin-fixed, paraffin-embedded, tissue obtained from amyloidotic mice and a post-mortem kidney specimen from a patient with amyloidosis secondary to osteomyelitis. Amyloidosis was induced in five out of 20 white mice by weekly intraperitoneal injections of complete Freund's adjuvant. Amyloid deposits were clearly demonstrated both by the Congo Red and by the IP techniques. The antibody against murine AA was found to be cross-reactive with human AA. These preliminary results suggest that the IP technique with the use of specific antibodies against the different amyloid proteins might be extremely useful for the histological dianosis and classification of amyloidosis.
一种特异性兔抗鼠淀粉样蛋白A蛋白（抗AA）已用于免疫过氧化物酶（IP）技术，以检测从淀粉样变性小鼠获得的福尔马林固定、石蜡包埋组织以及一名骨髓炎继发淀粉样变性患者的尸检肾脏标本中的淀粉样物质。通过每周腹腔注射完全弗氏佐剂，在20只白色小鼠中的5只诱导出淀粉样变性。刚果红和IP技术均清楚地显示出淀粉样沉积物。发现抗鼠AA抗体与人AA具有交叉反应性。这些初步结果表明，使用针对不同淀粉样蛋白的特异性抗体的IP技术可能对淀粉样变性的组织学诊断和分类极为有用。
