Mutzner P A, Stuhlmiller G M, Seigler H F
J Surg Oncol. 1980;14(4):367-77. doi: 10.1002/jso.2930140411.
Xenoantiserum, alloantiserum, and autoantiserum prepared against human melanoma cells from cell lines maintained in tissue culture were evaluated by indirect immunofluorescence (ind IF) for their content of antibodies reactive with melanoma cell membrane tumor-associated antigens (TAA). Following absorptions which removed all detectable anti-human species, anti-RBC, and anti-HLA antigen activity, the antisera were tested against a panel of human cell lines. Three apparently distinct melanoma-associated cell membrane antigens were detected. The first of these was present on all human cells maintained in tissue culture including cells of both malignant and nonmalignant origin. The second antigen was expressed on all cells of malignant origin and also by fetal fibroblasts. The third antigen was detectable only on melanoma cells and was expressed by a majority of the melanomas tested. No evidence was obtained to suggest the presence of melanoma TAA unique to a single melanoma cell line. Marked differences were observed in the antigenic profile detected by the three antisera.
通过间接免疫荧光法（ind IF）评估了针对组织培养中维持的人黑色素瘤细胞系制备的异种抗血清、同种抗血清和自身抗血清中与黑色素瘤细胞膜肿瘤相关抗原（TAA）反应的抗体含量。在去除所有可检测到的抗人抗体、抗红细胞抗体和抗HLA抗原活性后，对这些抗血清进行了一组人细胞系的检测。检测到三种明显不同的黑色素瘤相关细胞膜抗原。其中第一种存在于组织培养中维持的所有人类细胞上，包括恶性和非恶性来源的细胞。第二种抗原在所有恶性来源的细胞以及胎儿成纤维细胞上表达。第三种抗原仅在黑色素瘤细胞上可检测到，并且在所测试的大多数黑色素瘤中表达。没有证据表明存在单个黑色素瘤细胞系特有的黑色素瘤TAA。观察到三种抗血清检测到的抗原谱存在明显差异。
