卡托普利刺激脑啡肽降解。

Enkephalin degradation stimulated by captopril.

作者信息

Hui K S, Cheng K P, Banay-Schwartz M, Lajtha A

出版信息

Neurosci Lett. 1980 Dec;20(3):341-5. doi: 10.1016/0304-3940(80)90171-8.

Abstract

Changes in the rates of degradation of Met- and Leu-enkephalin by brain aminopeptidase were measured by UV absorption after high-pressure liquid chromatography. The cleavage of enkephalin paralleled the generation of tyrosine. Captopril (SQ 14,225) stimulated enzyme activity, to a greater extent with Met- than with Leu-enkephalin. Pro-His-Pro-NH2, histidine, and histamine stimulated enkephalin aminopeptidase approximately twice as much as captopril. The sulfhydryl group of captopril was not the only factor in its effect. The addition of captopril did not change the pH optimum, and it stimulated enkephalin degradation between 25 and 50 degrees C.

摘要

通过高压液相色谱法测定脑氨肽酶对甲硫氨酸脑啡肽和亮氨酸脑啡肽的降解速率变化，采用紫外吸收法进行检测。脑啡肽的裂解与酪氨酸的生成平行。卡托普利（SQ 14,225）刺激酶活性，对甲硫氨酸脑啡肽的刺激程度大于亮氨酸脑啡肽。脯氨酸-组氨酸-脯氨酸-氨基，组氨酸和组胺对脑啡肽氨肽酶的刺激作用约为卡托普利的两倍。卡托普利的巯基并非其发挥作用的唯一因素。添加卡托普利并未改变最适pH值，且在25至50摄氏度之间刺激脑啡肽降解。