通过差异氢交换表征Mg2+诱导的50S核糖体亚基构象变化
Characterization of Mg2+-induced conformational change in the 50S ribosomal subunit by differential hydrogen exchange.
作者信息
Bonnet D, Begard E, Grunberg-Manago M, Hui Bon Hoa G
出版信息
Nucleic Acids Res. 1980 Jun 11;8(11):2489-98. doi: 10.1093/nar/8.11.2489.
Abstract
The technique of differential hydrogen exchange allows detection of a conformational change in the 50S subunit of Escherichia coli ribosome when the magnesium concentration is lowered in a range where ribosomal activity is fully preserved. This change is characterized by a seventy-fold acceleration of about thirty labile hydrogens in the case of a Mg2+ jump from 10 mM to 2 mM. The small number of hydrogens involved can explain the difficulty in detecting this change by other methods.
摘要
差异氢交换技术能够检测到，当镁离子浓度在核糖体活性完全保持的范围内降低时，大肠杆菌核糖体50S亚基的构象变化。这种变化的特征是，在Mg2+从10 mM跃降至2 mM的情况下，约30个不稳定氢的交换速度加快了70倍。涉及的氢数量较少，可以解释通过其他方法检测这种变化的困难。
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