Klein H L, Welch S K
Nucleic Acids Res. 1980 Oct 24;8(20):4651-69. doi: 10.1093/nar/8.20.4651.
The inverted repeated sequences (foldback DNA) of yeast nuclear DNA have been examined by electron microscopy and hydroxyapatite chromatography. Of the inverted repeat structures seen in the electron microscope, 34% were hairpins and 66% had a single stranded loop at the end of a duplex stem. The number average length of the repeat was 0.3 kb and the single stranded loop was 1.6 kb. It is estimated that there are approximately 250 inverted repeats per haploid genome. A statistical analysis of the frequency of molecules containing multiple inverted repeats showed that these sequences are non-randomly distributed. The distribution of inverted repeats was also examined by measuring the fraction of total DNA in the foldback fraction that bound to hydroxyapatite as a function of single strand fragment size. This analysis also indicated that the inverted repeats are clustered. Renaturation kinetic analysis of isolated foldback and inverted repeat stem sequence DNA showed that these sequences are enriched for repetitive DNA.
已通过电子显微镜和羟基磷灰石色谱法对酵母核DNA的反向重复序列（回折DNA）进行了检测。在电子显微镜下观察到的反向重复结构中，34%为发夹结构，66%在双链茎的末端有一个单链环。重复序列的数均长度为0.3 kb，单链环为1.6 kb。据估计，每个单倍体基因组中约有250个反向重复序列。对含有多个反向重复序列的分子频率进行的统计分析表明，这些序列是非随机分布的。还通过测量作为单链片段大小函数的与羟基磷灰石结合的回折部分中总DNA的比例，来检测反向重复序列的分布。该分析也表明反向重复序列是成簇的。对分离出的回折和反向重复茎序列DNA的复性动力学分析表明，这些序列富含重复DNA。
