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1
Role of the 5'-terminal phosphate of tRNA for its function during protein biosynthesis elongation cycle.转运RNA的5'-末端磷酸在蛋白质生物合成延伸循环中的功能作用。
Nucleic Acids Res. 1980 Oct 24;8(20):4737-44. doi: 10.1093/nar/8.20.4737.
2
The complex formation between Escherichia coli aminoacyl-tRNA, elongation factor Tu and GTP. The effect of the side-chain of the amino acid linked to tRNA.大肠杆菌氨酰-tRNA、延伸因子Tu与GTP之间的复合物形成。与tRNA相连的氨基酸侧链的作用。
Eur J Biochem. 1980;108(1):213-21. doi: 10.1111/j.1432-1033.1980.tb04714.x.
3
Kirromycin, an inhibitor of protein biosynthesis that acts on elongation factor Tu.奇霉素，一种作用于延伸因子Tu的蛋白质生物合成抑制剂。
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4910-4. doi: 10.1073/pnas.71.12.4910.
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Hydrolysis of GTP on elongation factor Tu.ribosome complexes promoted by 2'(3')-O-L-phenylalanyladenosine.2'（3'）-O-L-苯丙氨酰腺苷促进延伸因子Tu-核糖体复合物上GTP的水解。
Proc Natl Acad Sci U S A. 1980 Feb;77(2):905-9. doi: 10.1073/pnas.77.2.905.
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GTP consumption of elongation factor Tu during translation of heteropolymeric mRNAs.异聚体mRNA翻译过程中延伸因子Tu的GTP消耗
Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):1945-9. doi: 10.1073/pnas.92.6.1945.
6
Pulvomycin, an inhibitor of protein biosynthesis preventing ternary complex formation between elongation factor Tu, GTP, and aminoacyl-tRNA.普尔沃霉素，一种蛋白质生物合成抑制剂，可阻止延伸因子Tu、GTP和氨酰tRNA之间形成三元复合物。
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5324-8. doi: 10.1073/pnas.75.11.5324.
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Aminoacyl-tRNA-elongation factor Tu-ribosome interaction leading to hydrolysis of guanosine 5'-triphosphate.氨酰基转移核糖核酸-延伸因子Tu-核糖体相互作用导致三磷酸鸟苷水解。
Biochemistry. 1986 Dec 16;25(25):8330-6. doi: 10.1021/bi00373a030.
8
Complete kinetic mechanism of elongation factor Tu-dependent binding of aminoacyl-tRNA to the A site of the E. coli ribosome.延伸因子Tu依赖的氨酰tRNA与大肠杆菌核糖体A位点结合的完整动力学机制。
EMBO J. 1998 Dec 15;17(24):7490-7. doi: 10.1093/emboj/17.24.7490.
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Interaction of elongation factor Tu with the aminoacyl transfer ribonucleic acid dimer Phe-tRNA-Glu-tRNA.延伸因子Tu与氨酰基转移核糖核酸二聚体苯丙氨酸-转运核糖核酸-谷氨酸-转运核糖核酸的相互作用。
Biochemistry. 1981 Jan 20;20(2):449-52. doi: 10.1021/bi00505a034.
10
Enacyloxin IIa, an inhibitor of protein biosynthesis that acts on elongation factor Tu and the ribosome.恩酰洛新IIa，一种作用于延伸因子Tu和核糖体的蛋白质生物合成抑制剂。
EMBO J. 1996 May 15;15(10):2604-11.

引用本文的文献

1
HPLC for at-line reaction monitoring and purification improves yield and purity of tRNA.用于在线反应监测和纯化的高效液相色谱法提高了转运核糖核酸的产量和纯度。
Front Mol Biosci. 2024 Sep 27;11:1443917. doi: 10.3389/fmolb.2024.1443917. eCollection 2024.
2
Analysis of the functional consequences of lethal mutations in mitochondrial translational elongation factors.线粒体翻译延伸因子致死突变的功能后果分析。
Biochim Biophys Acta. 2010 Jul-Aug;1802(7-8):692-8. doi: 10.1016/j.bbadis.2010.04.003. Epub 2010 May 6.
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A disease-causing point mutation in human mitochondrial tRNAMet rsults in tRNA misfolding leading to defects in translational initiation and elongation.人类线粒体tRNAMet中的致病点突变导致tRNA错误折叠，从而引发翻译起始和延伸缺陷。
J Biol Chem. 2008 Dec 5;283(49):34445-56. doi: 10.1074/jbc.M806992200. Epub 2008 Oct 3.
4
Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro.体外转录的未修饰酵母苯丙氨酸转移RNA的生化与物理特性
Proc Natl Acad Sci U S A. 1988 Feb;85(4):1033-7. doi: 10.1073/pnas.85.4.1033.

本文引用的文献

1
Primary structure of elongation factor Tu from Escherichia coli.来自大肠杆菌的延伸因子Tu的一级结构。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1326-30. doi: 10.1073/pnas.77.3.1326.
2
The complex formation between Escherichia coli aminoacyl-tRNA, elongation factor Tu and GTP. The effect of the side-chain of the amino acid linked to tRNA.大肠杆菌氨酰-tRNA、延伸因子Tu与GTP之间的复合物形成。与tRNA相连的氨基酸侧链的作用。
Eur J Biochem. 1980;108(1):213-21. doi: 10.1111/j.1432-1033.1980.tb04714.x.
3
Isolation and properties of tRNA nucleotidyl transferase from yeast.酵母中tRNA核苷酸转移酶的分离与性质
Eur J Biochem. 1971 Sep 24;22(2):166-72. doi: 10.1111/j.1432-1033.1971.tb01528.x.
4
Affinity elution as a purification method for aminoacyl-tRNA synthetases.亲和洗脱作为氨酰-tRNA合成酶的一种纯化方法。
Eur J Biochem. 1973 Apr 2;34(1):84-90. doi: 10.1111/j.1432-1033.1973.tb02731.x.
5
Studies on polypeptide elongation factors from Escherichia coli. II. Purification of factors Tu-guanosine diphosphate, Ts, and Tu-Ts, and crystallization of Tu-guanosine diphosphate and Tu-Ts.大肠杆菌多肽延伸因子的研究。II. 因子Tu-二磷酸鸟苷、Ts以及Tu-Ts的纯化，以及Tu-二磷酸鸟苷和Tu-Ts的结晶
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Large scale purification of tRNA ser , tRNA tyr and tRNA phe from Baker's yeast.从面包酵母中大规模纯化丝氨酸转运核糖核酸、酪氨酸转运核糖核酸和苯丙氨酸转运核糖核酸。
Hoppe Seylers Z Physiol Chem. 1972 Aug;353(8):1330-6. doi: 10.1515/bchm2.1972.353.2.1330.
7
Structural requirements for recognition of Escherichia coli initiator and non-initiator transfer ribonucleic acids by bacterial T factor.细菌T因子识别大肠杆菌起始和非起始转移核糖核酸的结构要求
J Biol Chem. 1974 Nov 25;249(22):7102-10.
8
Binding of complementary oligonucleotides to amino-acylated tRNAPhe from yeast.互补寡核苷酸与来自酵母的氨酰化苯丙氨酸转运RNA的结合。
Biochem Biophys Res Commun. 1976 Aug 23;71(4):1025-33. doi: 10.1016/0006-291x(76)90757-9.
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Enzymatic incorporation of ATP and CTP analogues into the 3' end of tRNA.将ATP和CTP类似物酶促掺入tRNA的3'末端。
Eur J Biochem. 1977 Dec;81(3):579-89. doi: 10.1111/j.1432-1033.1977.tb11985.x.
10
Proton nuclear magnetic resonance of minor nucleosides in yeast phenylalanine transfer ribonucleic acid. Conformational changes as a consequence of aminoacylation, removal of the Y base, and codon--anticodon interaction.酵母苯丙氨酸转移核糖核酸中稀有核苷的质子核磁共振。氨基酰化、Y碱基去除以及密码子-反密码子相互作用导致的构象变化。
Biochemistry. 1979 Jul 24;18(15):3189-99. doi: 10.1021/bi00582a001.

转运RNA的5'-末端磷酸在蛋白质生物合成延伸循环中的功能作用。

Role of the 5'-terminal phosphate of tRNA for its function during protein biosynthesis elongation cycle.

作者信息

Sprinzl M, Graeser E

出版信息

Nucleic Acids Res. 1980 Oct 24;8(20):4737-44. doi: 10.1093/nar/8.20.4737.

DOI:10.1093/nar/8.20.4737
PMID:7003543
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324383/
Abstract

The 5'-terminal phosphate of tRNAPhe from yeast was removed using tRNAPhe lacking its 3'-terminal adenosine. After regeneration of the C-C-A terminus this tRNA was investigated in following reactions: aminoacylation, spontaneous hydrolysis of the amino acid from aminoacyl-tRNA, aminoacyl-tRNA.EF-Tu.GTP ternary complex formation and poly(U)-dependent synthesis of poly(Phe). The absence of the 5'-terminal phosphate of Phe-tRNAPhe does not influence the rate of hydrolysis of the amino acid or the ability of this rRNA to participate in complex formation with EF-Tu.GTP. The translation of the polyuridylic acid is slightly inhibited whereas the rate and extent of the enzymatic aminoacylation is not affected.

摘要

使用缺少3'-末端腺苷的酵母苯丙氨酸tRNA（tRNAPhe）去除tRNAPhe的5'-末端磷酸。在C-C-A末端再生后，对该tRNA进行了以下反应研究：氨酰化、氨酰-tRNA中氨基酸的自发水解、氨酰-tRNA.EF-Tu.GTP三元复合物形成以及聚（U）依赖性聚（苯丙氨酸）合成。苯丙氨酰-tRNAPhe的5'-末端磷酸缺失不影响氨基酸的水解速率或该rRNA与EF-Tu.GTP形成复合物的能力。聚尿苷酸的翻译略有抑制，而酶促氨酰化的速率和程度不受影响。