转运RNA的5'-末端磷酸在蛋白质生物合成延伸循环中的功能作用。
Role of the 5'-terminal phosphate of tRNA for its function during protein biosynthesis elongation cycle.
作者信息
Sprinzl M, Graeser E
出版信息
Nucleic Acids Res. 1980 Oct 24;8(20):4737-44. doi: 10.1093/nar/8.20.4737.
Abstract
The 5'-terminal phosphate of tRNAPhe from yeast was removed using tRNAPhe lacking its 3'-terminal adenosine. After regeneration of the C-C-A terminus this tRNA was investigated in following reactions: aminoacylation, spontaneous hydrolysis of the amino acid from aminoacyl-tRNA, aminoacyl-tRNA.EF-Tu.GTP ternary complex formation and poly(U)-dependent synthesis of poly(Phe). The absence of the 5'-terminal phosphate of Phe-tRNAPhe does not influence the rate of hydrolysis of the amino acid or the ability of this rRNA to participate in complex formation with EF-Tu.GTP. The translation of the polyuridylic acid is slightly inhibited whereas the rate and extent of the enzymatic aminoacylation is not affected.
摘要
使用缺少3'-末端腺苷的酵母苯丙氨酸tRNA（tRNAPhe）去除tRNAPhe的5'-末端磷酸。在C-C-A末端再生后，对该tRNA进行了以下反应研究：氨酰化、氨酰-tRNA中氨基酸的自发水解、氨酰-tRNA.EF-Tu.GTP三元复合物形成以及聚（U）依赖性聚（苯丙氨酸）合成。苯丙氨酰-tRNAPhe的5'-末端磷酸缺失不影响氨基酸的水解速率或该rRNA与EF-Tu.GTP形成复合物的能力。聚尿苷酸的翻译略有抑制，而酶促氨酰化的速率和程度不受影响。
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