Wolf E, Pompe B
Z Gesamte Inn Med. 1980 Jul 1;35(13):561-4.
A exact judgment of bone and bone marrow tissue is based on an undecalcified treatment technique by means of plastics embedding and hard section microtomy. All hitherto existing routine techniques of plastics embedding of bone tissue used monomeres of methakrylate with addition of so-called emollient substances for polymerisation. Disadvantages of a stronger shrinking of the polymerisation product and uncontrolled, not always avoidable accelerations of the polymerisation process which have an essentially negative influence on the histological results should thus be avoided. A very exact observance of the treatment steps is here necessary. In the further development of our embedding method we turned to the polymethacrylates. The polymerisation adhesive Kalloplast R used in industry proved as the most suitable initial substance for the new method. The yielded results lie in a considerable shortening and further simplification of the embedding technique. The results are always well reproducible. Undesirable shrinking or polymerisation artefacts with impairment of the histological results do no more appear.
对骨骼和骨髓组织的准确判断基于一种通过塑料包埋和硬切片显微切割的未脱钙处理技术。迄今为止，所有现有的骨骼组织塑料包埋常规技术都使用甲基丙烯酸酯单体，并添加所谓的软化物质进行聚合。因此，应避免聚合产物收缩较强以及聚合过程不受控制且并非总能避免的加速，这些对组织学结果有本质上的负面影响。在此，必须非常精确地遵守处理步骤。在我们包埋方法的进一步发展中，我们转向了聚甲基丙烯酸酯。工业上使用的聚合粘合剂Kalloplast R被证明是新方法最合适的起始物质。所得到的结果是包埋技术显著缩短并进一步简化。结果总是具有良好的可重复性。不再出现对组织学结果有损害的不良收缩或聚合假象。
